Fluorescence Anisotropy Spectroscopy in Aptamer-based Biosensing
Eric Peyrin, Emmanuelle Fiore, Valérie Guieu, Sandrine Perrier, Corinne Ravelet
Département de Pharmacochimie Moléculaire, Université Grenoble Alpes, UMR 5063 CNRS, ICMG FR 2607, Campus universitaire, Saint-Martin d’Hères, France
The fluorescence anisotropy/polarization (FA/FP) spectroscopy is very popular in the biochemical/biophysical field for the determination of volume and flexibility of macromolecules, the analysis of ligand-receptor binding, the study of the membrane fluidity or the tissue imaging as well as in the analytical chemistry area for clinical, environmental and food control applications. The FA technique is known to be relatively insensitive to fluorescence fluctuation and photobleaching and directly applicable to complex biological media.
During the past decade, numerous aptamer FA analytical methodologies have been developed. Here, we will focus on the different mechanisms and transduction principles used in this particular field of the aptamer sensing platforms. Both size- and segmental motion-based approaches will be presented with diverse applications to the detection of either small or large target molecules. We will also address the recent mass-amplified methods designed to improve the FA response.